Eddie James
Background Information
Dr. James received his bachelor’s degree in Chemical Engineering from the University of Colorado. He subsequently attended Washington State University, receiving his PhD in Biochemical Engineering in 2001. After completing an internship at Zymogenetics, he joined the Benaroya Research Institute as manager of the tetramer core and is currently a BRI assistant member.
Area of Research
Dr. James leads several independent research projects related to the role of T cells in autoimmune disease. These projects include a JDRF funded study to investigate the role of protein modifications in the development of destructive immune responses in type 1 diabetes and a cooperative JDRF project aimed at understanding immunologic reasons why some patients with type 1 diabetes rapidly lose all ability to produce insulin while others retain some residual insulin secretion. He also collaborates with Dr. John Piganelli and Dr. Meghan Marre of the University of Pittsburgh and Dr. Chantal Mathieu and Lut Overbergh to investigate how beta cell stress contributes to the breakdown of tolerance in autoimmune diabetes and with Dr. Bill Kwok to study activated T cells in subjects at risk of developing type 1 diabetes. Dr. James is currently co-chair of the JDRF biomarker working group and co-leads the Immunology of Diabetes Society T Cell Workshop. Under the auspices of these groups, he leads biomarker development and validation projects aimed at improved disease staging of subjects who are diagnosed with type 1 diabetes.
Dr. James participates in collaborative projects with Dr. Jane Buckner and colleagues at the University of Colorado aimed at characterizing T cell responses in patients with rheumatoid arthritis and investigating mechanisms that promote the breakdown of tolerance in at risk subjects. He is also involved in various other collaborative research projects, applying tetramer technology to identify the chemical patterns recognized by T cells in desirable protective immune responses directed against viruses (such as influenza, tetanus, and chicken pox) and in undesirable destructive immune responses directed against therapeutic proteins (including insulin and Factor VIII) and self-proteins in diseases such as IBD and pemphigus vulgaris. The unifying theme of this work is that understanding the basics of immune recognition and the specifics of both immune protection and immune destruction will lead to better diagnostic tools and better therapies.
Featured Publications
- Guyer P, Seminova K, Lugar M, Manganaro A, Velarde de la Cruz EE, Hartley R, Smithmyer ME, Speake C, Bonifacio E, Kent SC, James EA. T cell and autoantibody recognition of nucleus-associated islet autoantigens in individuals with type 1 diabetes. Diabetologia. Jul 2025. PMID: 40490584
- Savvides CS, James EA, Buckner JH. Rheumatoid arthritis - the role of T cells in this complex systemic autoimmune disease. Curr Opin Immunol. Apr 2025. PMID: 40209552
- Hohenstein AC, Gallegos JB, Dang M, Groegler J, Broncucia H, Tensun FA, Waugh K, Dong F, James EA, Speake C, Steck AK, Rewers MJ, Gottlieb PA, Haskins K, Delong T, Baker RL. Novel T-Cell Reactivities to Hybrid Insulin Peptides in Islet Autoantibody-Positive At-Risk Individuals. Diabetes. Mar 2025. PMID: 39820647
- Rims C, Uchtenhagen H, Brooks K, Ng B, Posso SE, Carlin J, Kwok WW, Buckner JH, James EA. Antigen-specific T-cell frequency and phenotype mirrors disease activity in DRB1*04:04+ rheumatoid arthritis patients. Clin Exp Immunol. Nov 2024. PMID: 39492692
- Tewari R, Yang SJ, McClain ED, Hu A, Mortensen E, DeSchmidt A, Chen J, Kancharla A, Singh AK, James EA, Burman BE, Siddique A, Rawlings DJ, Patel C, Cerosaletti K, Buckner JH. Identification of a novel PDC-E2 epitope in primary biliary cholangitis: Application for engineered Treg therapy. J Autoimmun. Oct 2024. PMID: 39476446
- Holers VM, Demoruelle KM, Buckner JH, James EA, Firestein GS, Robinson WH, Steere AC, Zhang F, Norris JM, Kuhn KA, Deane KD. Distinct mucosal endotypes as initiators and drivers of rheumatoid arthritis. Nat Rev Rheumatol. Sep 2024. PMID: 39251771
- Groegler J, Callebaut A, James EA, Delong T. The insulin secretory granule is a hotspot for autoantigen formation in type 1 diabetes. Diabetologia. May 2024. PMID: 38811417
- Karataş L, Tatar Z, James EA, Colakogullari M. Investigating Associations between HLA-DR Genotype, H. pylori Infection, and Anti-CagA IgA Seropositivity in a Turkish Gastritis Cohort. Genes (Basel). Mar 2024. PMID: 38540398
- Callebaut A, Guyer P, Derua R, Buitinga M, Manganaro A, Yi X, Sodré FMC, Vig S, Suleiman M, Marchetti P, Eizirik DL, Kent SC, Mathieu C, James EA, Overbergh L. CD4+ T cells from individuals with type 1 diabetes respond to a novel class of deamidated peptides formed in pancreatic islets. Diabetes. Feb 2024. PMID: 38387030
- Callebaut A, Guyer P, Baker RL, Gallegos JB, Hohenstein AC, Gottlieb PA, Mathieu C, Overbergh L, Haskins K, James EA. An Insulin-Chromogranin A Hybrid Peptide activates DR11 restricted T cells in human type 1 diabetes. Diabetes. Jan 2024. PMID: 38295386
- James EA, Holers VM, Iyer R, Prideaux EB, Rao NL, Rims C, Muir VS, Posso SE, Bloom MS, Zia A, Elliott SE, Adamska JZ, Ai R, Brewer RC, Seifert JA, Moss L, Barzideh S, Demoruelle MK, Striebich CC, Okamoto Y, Sainbayar E, Crook AA, Peterson RA, Vanderlinden LA, Wang W, Boyle DL, Robinson WH, Buckner JH, Firestein GS, Deane KD. Multifaceted immune dysregulation characterizes individuals at-risk for rheumatoid arthritis. Nat Commun. Nov 2023. PMID: 37993439
- James EA, Joglekar AV, Linnemann AK, Russ HA, Kent SC. The Beta Cell-Immune Cell Interface in Type 1 Diabetes (T1D). Mol Metab. Sep 2023. PMID: 37734713
- James EA. Editorial: Processing, presentation, and recognition of T cell determinants: From molecular insights to clinical applications. Cell Immunol. Aug 2023. PMID: 37603955
- Ettinger RA, Buitinga M, Vandamme C, Afonso G, Gomez R, Arribas-Layton D, Bissenova S, Speake C, Reijonen H, Kinnunen T, Overbergh L, Mallone R, Kwok WW, James EA. Technical Validation and Utility of an HLA Class II Tetramer Assay for Type 1 Diabetes: A Multicenter Study. J Clin Endocrinol Metab. Jul 2023. PMID: 37474341
- Moustakas AK, Nguyen H, James EA, Papadopoulos GK. Autoimmune susceptible HLA class II motifs facilitate the presentation of modified neoepitopes to potentially autoreactive T cells. Cell Immunol. May 2023. PMID: 37301094
- Moon JS, Younis S, Ramadoss NS, Iyer R, Sheth K, Sharpe O, Rao NL, Becart S, Carman JA, James EA, Buckner JH, Deane KD, Holers VM, Goodman SM, Donlin LT, Davis MM, Robinson WH. Cytotoxic CD8(+) T cells target citrullinated antigens in rheumatoid arthritis. Nat Commun. Jan 2023. PMID: 36658110
- Nguyen H, Arribas-Layton D, Chow IT, Speake C, Kwok WW, Hessner MJ, Greenbaum CJ, James EA. Characterizing T cell responses to enzymatically modified beta cell neo-epitopes. Front Immunol. Jan 2023. PMID: 36703975
- Chriswell ME, Lefferts AR, Clay MR, Hsu AR, Seifert J, Feser ML, Rims C, Bloom MS, Bemis EA, Liu S, Maerz MD, Frank DN, Demoruelle MK, Deane KD, James EA, Buckner JH, Robinson WH, Holers VM, Kuhn KA. Clonal IgA and IgG autoantibodies from individuals at risk for rheumatoid arthritis identify an arthritogenic strain of Subdoligranulum. Sci Transl Med. Oct 2022. PMID: 36288282
- Strollo R, Vinci C, Man YKS, Bruzzaniti S, Piemonte E, Alhamar G, Briganti SI, Malandrucco I, Tramontana F, Fanali C, Garnett J, Buccafusca R, Guyer P, Mamula M, James EA, Pozzilli P, Ludvigsson J, Winyard PG, Galgani M, Nissim A. Autoantibody and T cell responses to oxidative post-translationally modified insulin neoantigenic peptides in type 1 diabetes. Diabetologia. Oct 2022. PMID: 36207582
- Guyer P, Arribas-Layton D, Manganaro A, Speake C, Lord S, Eizirik DL, Kent SC, Mallone R, James EA. Recognition of mRNA Splice Variant and Secretory Granule Epitopes by CD4+ T Cells in Type 1 Diabetes. Diabetes. Oct 2022. PMID: 36201618
- Alvarez I, Antón LC, James EA. Editorial: alternative antigen processing and presentation in immune disorders. Front Immunol. Sep 2022. PMID: 36159790
- Holers VM, Kuhn KA, Demoruelle MK, Norris JM, Firestein GS, James EA, Robinson WH, Buckner JH, Deane KD. Mechanism-driven strategies for prevention of rheumatoid arthritis. Rheumatol Autoimmun. Sep 2022. PMID: 36312783
- Yang ML, Connolly SE, Gee RJ, Lam TT, Kanyo J, Peng J, Guyer P, Syed F, Tse HM, Clarke SG, Clarke CF, James EA, Speake C, Evans-Molina C, Arvan P, Herold KC, Wen L, Mamula MJ. Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. Diabetes. Sep 2022. PMID: 35730902
- Chen D, McInnis E, Wu E, Stember K, Hogan S, Hu Y, Henderson C, Blazek L, Mallal S, Karosiene E, Peters B, Sidney J, James EA, Kwok WW, Jennette JC, Ciavatta DJ, Falk RJ, Free ME. Immunological Interaction of HLA-DPB1 and Proteinase 3 in ANCA Vasculitis is Associated with Clinical Disease Activity. J Am Soc Nephrol. Jun 2022. PMID: 35672132
- Maggi J, Carrascal M, Soto L, Neira O, Cuéllar MC, Aravena O, James EA, Abian J, Jaraquemada D, Catalan D, Aguillón JC. Isolation of HLA-DR-naturally presented peptides identifies T-cell epitopes for rheumatoid arthritis. Ann Rheum Dis. Apr 2022. PMID: 35459695
- Yang ML, Horstman S, Gee R, Guyer P, Lam TT, Kanyo J, Perdigoto AL, Speake C, Greenbaum CJ, Callebaut A, Overbergh L, Kibbey RG, Herold KC, James EA, Mamula MJ. Citrullination of glucokinase is linked to autoimmune diabetes. Nat Commun. Apr 2022. PMID: 35388005
- Ishigaki K, Lagattuta KA, Luo Y, James EA, Buckner JH, Raychaudhuri S. HLA autoimmune risk alleles restrict the hypervariable region of T cell receptors. Nat Genet. Mar 2022. PMID: 35332318
- Jing Y, Kong Y, McGinty J, Blahnik-Fagan G, Lee T, Orozco-Figueroa S, Bettini ML, James EA, Bettini M. TCR/HLA Humanized Mice Reveal Reduced Tolerance and Increased Immunogenicity of Post-translationally Modified GAD65 Epitope. Diabetes. Feb 2022. PMID: 35179565
- Yang JHM, Ward-Hartstonge KA, Perry DJ, Blanchfield JL, Posgai AL, Wiedeman AE, Diggins K, Rahman A, Tree TIM, Brusko TM, Levings MK, James EA, Kent SC, Speake C, Homann D, Long SA, Immunology of Diabetes Society T-Cell Cytometry Group.. Guidelines for standardizing T-cell cytometry assays to link biomarkers, mechanisms, and disease outcomes in type 1 diabetes. Eur J Immunol. Jan 2022. PMID: 35025103
- Hill DL, Whyte CE, Innocentin S, Lee JL, Dooley J, Wang J, James EA, Lee JC, Kwok WW, Zand MS, Liston A, Carr EJ, Linterman MA. Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans. Elife. Nov 2021. PMID: 34726156
- Rodriguez-Calvo T, Christoffersson G, Bender C, von Herrath MG, Mallone R, Kent SC, James EA. Means, Motive, and Opportunity: Do Non-Islet-Reactive Infiltrating T Cells Contribute to Autoimmunity in Type 1 Diabetes? Front Immunol. Jun 2021. PMID: 34220832)
- Wiles TA, Hohenstein A, Landry LG, Dang M, Powell R, Guyer P, James EA, Nakayama M, Haskins K, Delong T, Baker RL. Characterization of Human CD4 T Cells Specific for a C-Peptide/C-Peptide Hybrid Insulin Peptide. Front Immunol. May 2021. PMID: 34113344
- Song J, Schwenzer A, Wong A, Turcinov S, Rims C, Martinez LR, Arribas-Layton D, Gerstner C, Muir VS, Midwood KS, Malmström V, James EA, Buckner JH. Shared recognition of citrullinated tenascin-C peptides by T and B cells in rheumatoid arthritis. JCI Insight. Mar 2021. PMID: 33507879
- Piganelli JD, Mamula MJ, James EA. The Role of β Cell Stress and Neo-Epitopes in the Immunopathology of Type 1 Diabetes. Front Endocrinol (Lausanne). Feb 2021. PMID: 33679609
- Nguyen H, Guyer P, Ettinger RA, James EA. Non-Genetically Encoded Epitopes Are Relevant Targets in Autoimmune Diabetes. Biomedicines. Feb 2021. PMID: 33671312
- DeGottardi Q, Gates TJ, Yang J, James EA, Malhotra U, Chow IT, Simoni Y, Fehlings M, Newell EW, DeBerg HA, Kwok WW. Ontogeny of different subsets of yellow fever virus-specific circulatory CXCR5+ CD4+ T cells after yellow fever vaccination. Sci Rep. Sep 2020. PMID: 32973217
- Carmona-Rivera C, Carlucci PM, Goel RR, James EA, Brooks SR, Rims C, Hoffmann V, Fox DA, Buckner JH, Kaplan MJ. Neutrophil extracellular traps mediate articular cartilage damage and enhance cartilage component immunogenicity in rheumatoid arthritis. JCI Insight. Jul 2020. PMID: 32484790
- James EA, Mallone R, Kent SC, DiLorenzo TP. T-Cell Epitopes and Neo-epitopes in Type 1 Diabetes: A Comprehensive Update and Reappraisal. Diabetes. Jul 2020. PMID: 32561620
- Arribas-Layton D, Guyer P, Delong T, Dang M, Chow IT, Speake C, Greenbaum CJ, Kwok WW, Baker RL, Haskins K, James EA. Hybrid Insulin Peptides Are Recognized by Human T Cells in the Context of DRB1*04:01. Diabetes. Jul 2020. PMID: 32291282
- Larson D, Patel P, Salapatek AM, Couroux P, Whitehouse D, Pina A, Johnson JL, Sever ML, Sanda S, Poyser J, Allio T, Scadding GW, Qin T, Shamji MH, Kwok WW, James EA, French D, Lelic A, Larché M, Altman MC, Togias A, Durham SR. Nasal allergen challenge and environmental exposure chamber challenge: A randomized trial comparing clinical and biological responses to cat allergen. J Allergy Clin Immunol. Jun 2020. PMID: 32169380
- Abdelsamed HA, Zebley CC, Nguyen H, Rutishauser RL, Fan Y, Ghoneim HE, Crawford JC, Alfei F, Alli S, Ribeiro SP, Castellaw AH, McGargill MA, Jin H, Boi SK, Speake C, Serti E, Turka LA, Busch ME, Stone M, Deeks SG, Sekaly RP, Zehn D, James EA, Nepom GT, Youngblood B. Beta cell-specific CD8+ T cells maintain stem cell memory-associated epigenetic programs during type 1 diabetes. Nat Immunol. May 2020. PMID: 32231298
- Lande R, Palazzo R, Gestermann N, Jandus C, Falchi M, Spadaro F, Riccieri V, James EA, Butera A, Boirivant M, Feldmeyer L, Surbeck I, Di Lucca J, Stuber F, Spinelli FR, Botti E, Marinari B, Bianchi L, Pica R, Cerbelli B, Giannakakis K, Auteri SE, Daniels I, Durrant LG, Horstman S, Costanzo A, Romero P, Alessandri C, Conti F, Valesini G, Gilliet M, Chizzolini C, Frasca L. Native/citrullinated LL37-specific T-cells help autoantibody production in Systemic Lupus Erythematosus. Sci Rep. Apr 2020. PMID: 32245990
- Wen X, Yang J, James EA, Chow IT , Reijonen H , Kwok WW. Increased islet antigen-specific regulatory and effector CD4+ T cells in healthy individuals with the type 1 diabetes-protective haplotype. Sci Immunol. Feb 2020. PMID: 32060144
- Wiedeman AE, Muir VS, Rosasco MG, DeBerg HA, Presnell S, Haas B, Dufort MJ, Speake C, Greenbaum CJ, Serti E, Nepom GT, Blahnik G, Kus AM, James EA, Linsley PS, Long SA. Autoreactive CD8+ T cell exhaustion distinguishes subjects with slow type 1 diabetes progression. J Clin Invest. Jan 2020. PMID: 31815738
- Free ME, Stember KG, Hess JJ, McInnis EA, Lardinois O, Hogan SL, Hu Y, Mendoza C, Le AK, Guseman AJ, Pilkinton MA, Bortone DS, Cowens K, Sidney J, Karosiene E, Peters B, James EA, Kwok WW, Vincent BG, Mallal SA, Jennette JC, Ciavatta DJ, Falk RJ. Restricted myeloperoxidase epitopes drive the adaptive immune response in MPO-ANCA vasculitis. J Autoimmun. Jan 2020. PMID: 31383567
- Uchida AM, Boden EK, James EA, Shows DM, Konecny AJ, Lord JD. Escherichiacoli-Specific CD4+ T Cells Have Public T-Cell Receptors and Low Interleukin 10 Production in Crohn's Disease. Cell Mol Gastroenterol Hepatol. Jan 2020. PMID: 32361018
- Galindo-Feria AS, Albrecht I, Fernandes-Cerqueira C, Notarnicola A, James EA, Herrath J, Dastmalchi M, Sandalova T, Rönnblom L, Jakobsson PJ, Fathi M, Achour A, Grunewald J, Malmström V, Lundberg IE. Proinflammatory Histidyl-Transfer RNA Synthetase-Specific CD4+ T Cells in the Blood and Lungs of Patients With Idiopathic Inflammatory Myopathies. Arthritis Rheumatol. Jan 2020. PMID: 31403245
- Speake C, Skinner SO, Berel D, Whalen E, Dufort MJ, Young WC, Odegard JM, Pesenacker AM, Gorus FK, James EA, Levings MK, Linsley PS, Akirav EM, Pugliese A, Hessner MJ, Nepom GT, Gottardo R, Long SA. A composite immune signature parallels disease progression across T1D subjects. JCI Insight. Dec 2019. PMID: 31671072
- Rudulier CD, Tonti E, James EA, Kwok WW, Larché M. Modulation of CRTh2 expression on allergen-specific T cells following peptide immunotherapy. Allergy. Nov 2019. PMID: 31077596
- Longino NV, Yang J, Iyer JG, Ibrani D, Chow IT, Laing KJ, Campbell VL, Paulson KG, Kulikauskas RM, Church CD, James EA, Nghiem P, Kwok WW, Koelle DM. Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain. Cancer Immunol Res. Oct 2019. PMID: 31405946
- Meckiff BJ, Ladell K, McLaren JE, Ryan GB, Leese AM, James EA, Price DA, Long HM. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. Sep 2019. PMID: 31308093
- Chow IT, Gates TJ, Papadopoulos GK, Moustakas AK, Kolawole EM, Notturno RJ, McGinty JW, Torres-Chinn N, James EA, Greenbaum CJ, Nepom GT, Kwok WW. Discriminative T cell recognition of cross-reactive islet-antigens is associated with HLA-DQ8 transdimer-mediated autoimmune diabetes. Sci Adv. Aug 2019. PMID: 31457096
- Ahmed S, Cerosaletti K, James EA, Long SA, Mannering SI, Speake C, Nakayama M, Tree T, Roep BO, Herold KC, Brusko TM. Standardizing T-Cell Biomarkers in Type 1 Diabetes: Challenges and Recent Advances. Diabetes. Jul 2019. PMID: 31221801
- Rims C, Uchtenhagen H, Kaplan MJ, Carmona-Rivera C, Carlucci P, Mikecz K, Markovics A, Carlin J, Buckner JH, James EA. Citrullinated Aggrecan Epitopes as Targets of Autoreactive CD4+ T Cells in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. Apr 2019. PMID: 30390384
- Blahnik G, Uchtenhagen H, Chow IT, Speake C, Greenbaum CJ, Kwok WW, James EA. Analysis of pancreatic beta cell specific CD4+ T cells reveals a predominance of proinsulin specific cells. Cell Immunol. Jan 2019. PMID: 30428974
- James EA, Gillette L, Durinovic-Belló I, Speake C, Bondinas GP, Moustakas AK, Greenbaum CJ, Papadopoulos GK, Kwok WW. DRB4*01:01 Has a Distinct Motif and Presents a Proinsulin Epitope That Is Recognized in Subjects with Type 1 Diabetes. J Immunol. Dec 2018. PMID: 30455401
- Yang J, Jing L, James EA, Gebe JA, Koelle DM, Kwok WW. A Novel Approach of Identifying Immunodominant Self and Viral Antigen Cross-Reactive T Cells and Defining the Epitopes They Recognize. Front Immunol. Dec 2018. PMID: 30619245
- Buitinga M, Callebaut A, Marques Câmara Sodré F, Crèvecoeur I, Blahnik-Fagan G, Yang ML, Bugliani M, Arribas-Layton D, Marré M, Cook DP, Waelkens E, Mallone R, Piganelli JD, Marchetti P, Mamula MJ, Derua R, James EA, Mathieu C, Overbergh L. Inflammation-Induced Citrullinated Glucose-Regulated Protein 78 Elicits Immune Responses in Human Type 1 Diabetes. Diabetes. Nov 2018. PMID: 30348823
- Birrueta G, Tripple V, Pham J, Manohar M, James EA, Kwok WW, Nadeau KC, Sette A, Peters B, Schulten V. Peanut-specific T cell responses in patients with different clinical reactivity. PLoS One. Oct 2018. PMID: 30304054
- Kimura T, Kobiyama K, Winkels H, Tse K, Miller J, Vassallo M, Wolf D, Ryden C, Orecchioni M, Dileepan T, Jenkins MK, James EA, Kwok WW, Hanna DB, Kaplan RC, Strickler HD, Durkin HG, Kassaye SG, Karim R, Tien PC, Landay AL, Gange SJ, Sidney J, Sette A, Ley K. Regulatory CD4+ T Cells Recognize Major Histocompatibility Complex Class II Molecule-Restricted Peptide Epitopes of Apolipoprotein B. Circulation. Sep 2018. PMID: 29588316
- Marré ML, Piganelli JD, James EA. Protecting functional β cells with a therapeutic peptide. Ann Transl Med. Sep 2018. PMID: 30370299
- Pieper J, Dubnovitsky A, Gerstner C, James EA, Rieck M, Kozhukh G, Tandre K, Pellegrino S, Gebe JA, Rönnblom L, Sandalova T, Kwok WW, Klareskog L, Buckner JH, Achour A, Malmström V. Memory T cells specific to citrullinated α-enolase are enriched in the rheumatic joint. J Autoimmun. Aug 2018. PMID: 29853344
- Marré ML, McGinty JW, Chow IT, DeNicola ME, Beck NW, Kent SC, Powers AC, Bottino R, Harlan DM, Greenbaum CJ, Kwok WW, Piganelli JD, James EA. Modifying Enzymes Are Elicited by ER Stress, Generating Epitopes That Are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes. Diabetes. Jul 2018. PMID: 29654212
- James EA, Pietropaolo M, Mamula MJ. Immune Recognition of β-Cells: Neoepitopes as Key Players in the Loss of Tolerance. Diabetes. Jun 2018. PMID: 29784651
- James EA, Abreu JRF, McGinty JW, Odegard JM, Fillié YE, Hocter CN, Culina S, Ladell K, Price DA, Alkanani A, Rihanek M, Fitzgerald-Miller L, Skowera A, Speake C, Gottlieb P, Davidson HW, Wong FS, Roep B, Mallone R, Immunology of Diabetes Society T Cell Workshop Committee.. Combinatorial detection of autoreactive CD8+ T cells with HLA-A2 multimers: a multi-centre study by the Immunology of Diabetes Society T Cell Workshop. Diabetologia. Mar 2018. PMID: 29196783
- Ettinger RA, Liberman JA, Gunasekera D, Puranik K, James EA, Thompson AR, Pratt KP. FVIII proteins with a modified immunodominant T-cell epitope exhibit reduced immunogenicity and normal FVIII activity. Blood Adv. Feb 2018. PMID: 29444872
- Reynolds CJ, Suleyman OM, Ortega-Prieto AM, Skelton JK, Bonnesoeur P, Blohm A, Carregaro V, Silva JS, James EA, Maillère B, Dorner M, Boyton RJ, Altmann DM. T cell immunity to Zika virus targets immunodominant epitopes that show cross-reactivity with other Flaviviruses. Sci Rep. Jan 2018. PMID: 29330423
- James EA. Direct Analysis of Insulin-Specific T Cells Provides New Insights. Diabetes. Dec 2017. PMID: 29162735
- Sprouse ML, Blahnik G, Lee T, Tully N, Benarjee P, James EA, Redondo MJ, Bettini ML, Bettini M. Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs. J Vis Exp. Sep 2017. PMID: 28930975
- Scholz EM, Marcilla M, Daura X, Arribas-Layton D, James EA, Alvarez I. Human Leukocyte Antigen (HLA)-DRB1*15:01 and HLA-DRB5*01:01 Present Complementary Peptide Repertoires. Front Immunol. Aug 2017. PMID: 28871256
- Acevedo-Calado M, James EA, Morran MP, Pietropaolo SL, Ouyang Q, Arribas-Layton D, Songini M, Liguori M, Casu A, Auchus RJ, Huang S, Yu L, Michels A, Gianani R, Pietropaolo M. Identification of Unique Antigenic Determinants in the Amino Terminus of IA-2 (ICA512) in Childhood and Adult Autoimmune Diabetes: New Biomarker Development. Diabetes Care. Apr 2017. PMID: 28174261
- Carmona-Rivera C, Carlucci PM, Moore E, Lingampalli N, Uchtenhagen H, James EA, Liu Y, Bicker KL, Wahamaa H, Hoffmann V, Catrina AI, Thompson PR, Buckner JH, Robinson WH, Fox DA, Kaplan MJ. Synovial fibroblast-neutrophil interactions promote pathogenic adaptive immunity in rheumatoid arthritis. Sci Immunol. Apr 2017. PMID: 28649674
- Sprouse ML, Blahnik G, Lee T, Tully N, Banerjee P, James EA, Redondo MJ, Bettini ML, Bettini M. Rapid identification and expression of human TCRs in retrogenic mice. J Immunol Methods. Dec 2016. PMID: 27589924
- Babon JA, DeNicola ME, Blodgett DM, Crèvecoeur I, Buttrick TS, Maehr R, Bottino R, Naji A, Kaddis J, Elyaman W, James EA, Haliyur R, Brissova M, Overbergh L, Mathieu C, Delong T, Haskins K, Pugliese A, Campbell-Thompson M, Mathews C, Atkinson MA, Powers AC, Harlan DM, Kent SC. Analysis of self-antigen specificity of islet-infiltrating T cells from human donors with type 1 diabetes. Nat Med. Dec 2016. PMID: 27798614
- Gerstner C, Dubnovitsky A, Sandin C, Kozhukh G, Uchtenhagen H, James EA, Rönnelid J, Ytterberg AJ, Pieper J, Reed E, Tandre K, Rieck M, Zubarev RA, Rönnblom L, Sandalova T, Buckner JH, Achour A, Malmström V. Functional and Structural Characterization of a Novel HLA-DRB1*04:01-Restricted α-Enolase T Cell Epitope in Rheumatoid Arthritis. Front Immunol. Nov 2016. PMID: 27895642
- Ettinger RA, Paz P, James EA, Gunasekera D, Aswad F, Thompson AR, Matthews DC, Pratt KP. T cells from hemophilia A subjects recognize the same HLA-restricted FVIII epitope with a narrow TCR repertoire. Blood. Oct 2016. PMID: 27471234
- Scholz E, Mestre-Ferrer A, Daura X, García-Medel N, Carrascal M, James EA, Kwok WW, Canals F, Alvarez I. A Comparative Analysis of the Peptide Repertoires of HLA-DR Molecules Differentially Associated With Rheumatoid Arthritis. Arthritis Rheumatol. Oct 2016. PMID: 27158783
- Nguyen H, James EA. Immune recognition of citrullinated epitopes. Immunology. Oct 2016. PMID: 27531825
- Uchtenhagen H, Rims C, Blahnik G, Chow IT, Kwok WW, Buckner JH, James EA. Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining. Nat Commun. Aug 2016. PMID: 27571776
- Hinz D, Seumois G, Gholami AM, Greenbaum JA, Lane J, White B, Broide DH, Schulten V, Sidney J, Bakhru P, Oseroff C, Wambre E, James EA, Kwok WW, Peters B, Vijayanand P, Sette A. Lack of allergy to timothy grass pollen is not a passive phenomenon but associated with the allergen-specific modulation of immune reactivity. Clin Exp Allergy. May 2016. PMID: 26662458
- Clemente-Casares X, Blanco J, Ambalavanan P, Yamanouchi J, Singha S, Fandos C, Tsai S, Wang J, Garabatos N, Izquierdo C, Agrawal S, Keough MB, Yong VW, James EA, Moore A , Yang Y , Stratmann T , Serra P , Santamaria P. Expanding antigen-specific regulatory networks to treat autoimmunity. Nature. Feb 2016. PMID: 26886799
- James EA, Gates TJ, LaFond RE, Yamamoto S, Ni C, Mai D, Gersuk VH, O'Brien K, Nguyen QA, Zeitner B, Lanteri MC, Norris PJ, Chaussabel D, Malhotra U, Kwok WW. Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome. PLoS Pathog. Jan 2016. PMID: 26795118
- McGinty JW, Marré ML, Bajzik V, Piganelli JD, James EA. T cell epitopes and post-translationally modified epitopes in type 1 diabetes. Curr Diab Rep. Nov 2015. PMID: 26370701
- Marré ML, James EA, Piganelli JD. β cell ER stress and the implications for immunogenicity in type 1 diabetes. Front Cell Dev Biol. Oct 2015. PMID: 26579520
- Gunasekera D, Ettinger RA, Nakaya Fletcher S, James EA, Liu M, Barrett JC, Withycombe J, Matthews DC, Epstein MS, Hughes RJ, Pratt KP, Personalized Approaches to Therapies for Hemophilia (PATH) Study Investigators.. Factor VIII gene variants and inhibitor risk in African American hemophilia A patients. Blood. Aug 2015. PMID: 25617427
- Alvarez I, Collado JA, Colobran R, Carrascal M, Ciudad MT, Canals F, James EA, Kwok WW, Gärtner M, Kyewski B, Pujol-Borrell R, Jaraquemada D. Central T cell tolerance: Identification of tissue-restricted autoantigens in the thymus HLA-DR peptidome. J Autoimmun. Jun 2015. PMID: 25911201
- Chow IT, Yang J, Gates TJ, James EA, Mai DT, Greenbaum CJ, Kwok WW. Assessment of CD4+ T cell responses to glutamic acid decarboxylase 65 using DQ8 tetramers reveals a pathogenic role of GAD65 121-140 and GAD65 250-266 in T1D development. PLoS One. Nov 2014. PMID: 25405480
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- Archila LD, DeLong JH, Wambre E, James EA, Robinson DM, Kwok WW. Grass-specific CD4(+) T-cells exhibit varying degrees of cross-reactivity, implications for allergen-specific immunotherapy. Clin Exp Allergy. Jul 2014. PMID: 24708411
- James EA, Rieck M, Pieper J, Gebe JA, Yue BB, Tatum M, Peda M, Sandin C, Klareskog L, Malmström V, Buckner JH. Citrulline-specific Th1 cells are increased in rheumatoid arthritis and their frequency is influenced by disease duration and therapy. Arthritis Rheumatol. Jul 2014. PMID: 24665079
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- James EA, LaFond RE, Gates TJ, Mai DT, Malhotra U, Kwok WW. Yellow fever vaccination elicits broad functional CD4+ T cell responses that recognize structural and nonstructural proteins. J Virol. Dec 2013. PMID: 24049183
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- Lindestam Arlehamn CS, Gerasimova A, Mele F, Henderson R, Swann J, Greenbaum JA, Kim Y, Sidney J, James EA, Taplitz R, McKinney DM, Kwok WW, Grey H, Sallusto F, Peters B, Sette A. Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset. PLoS Pathog. Jan 2013. PMID: 23358848
- Wambre E, James EA, Kwok WW. Characterization of CD4+ T cell subsets in allergy. Curr Opin Immunol. Dec 2012. PMID: 22889592
- Arlehamn CS, Sidney J, Henderson R, Greenbaum JA, James EA, Moutaftsi M, Coler R, McKinney DM, Park D, Taplitz R, Kwok WW, Grey H, Peters B, Sette A. Dissecting mechanisms of immunodominance to the common tuberculosis antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7), and Rv1038c (EsxJ). J Immunol. May 2012. PMID: 22504645
- Kwok WW, Tan V, Gillette L, Littell CT, Soltis MA, LaFond RB, Yang J, James EA, DeLong JH. Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire. J Immunol. Mar 2012. PMID: 22327072
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- James EA, Mallone R, Schloot NC, Gagnerault MC, Thorpe J, Fitzgerald-Miller L, Reichow J, Wagner R, Pham MN, Jospe N, Lou O, Gottlieb PA, Brooks-Worrell BM, Durinovic-Belló I, T-Cell Workshop Committee, Immunology of Diabetes Society.. Immunology of Diabetes Society T-Cell Workshop: HLA class II tetramer-directed epitope validation initiative. Diabetes Metab Res Rev. Nov 2011. PMID: 22069252
- Mallone R, Scotto M, Janicki CN, James EA, Fitzgerald-Miller L, Wagner R, Gottlieb P, Thorpe J, Jospe N, Durinovic-Belló I, Boitard C, Lou O, Dayan CM, Wong FS, T-Cell Workshop Committee, Immunology of Diabetes Society. Immunology of Diabetes Society T-Cell Workshop: HLA class I tetramer-directed epitope validation initiative T-Cell Workshop Report-HLA Class I Tetramer Validation Initiative. Diabetes Metab Res Rev. Nov 2011. PMID: 22069251
- Kotturi MF, Swann JA, Peters B, Arlehamn CL, Sidney J, Kolla RV, James EA, Akondy RS, Ahmed R, Kwok WW, Buchmeier MJ, Sette A. Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection. J Virol. Nov 2011. PMID: 21900169
- Mattapallil MJ, Silver PB, Mattapallil JJ, Horai R, Karabekian Z, McDowell JH, Chan CC, James EA, Kwok WW, Sen HN, Nussenblatt RB, David CS, Caspi RR. Uveitis-associated epitopes of retinal antigens are pathogenic in the humanized mouse model of uveitis and identify autoaggressive T cells. J Immunol. Aug 2011. PMID: 21765017
- James EA, Kwok WW. Autoreactive CD4(+) T cells in patients with atopic dermatitis. J Allergy Clin Immunol. Jul 2011. PMID: 21620451
- James EA, DeVoti JA, Rosenthal DW, Hatam LJ, Steinberg BM, Abramson AL, Kwok WW, Bonagura VR. Papillomavirus-specific CD4+ T cells exhibit reduced STAT-5 signaling and altered cytokine profiles in patients with recurrent respiratory papillomatosis. J Immunol. Jun 2011. PMID: 21531896
- DeLong JH, Simpson KH, Wambre E, James EA, Robinson D, Kwok WW. Ara h 1-reactive T cells in individuals with peanut allergy. J Allergy Clin Immunol. May 2011. PMID: 21459424
- James EA, van Haren SD, Ettinger RA, Fijnvandraat K, Liberman JA, Kwok WW, Voorberg J, Pratt KP. T-cell responses in two unrelated hemophilia A inhibitor subjects include an epitope at the factor VIII R593C missense site. J Thromb Haemost. Apr 2011. PMID: 21251204
- Ge X, James EA, Reijonen H, Kwok WW. Differences in self-peptide binding between T1D-related susceptible and protective DR4 subtypes. J Autoimmun. Mar 2011. PMID: 21257290
- James EA, Moustakas AK, Bui J, Papadopoulos GK, Bondinas G, Buckner JH, Kwok WW. HLA-DR1001 presents "altered-self" peptides derived from joint-associated proteins by accepting citrulline in three of its binding pockets. Arthritis Rheum. Oct 2010. PMID: 20533291
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- Ettinger RA, James EA, Kwok WW, Thompson AR, Pratt KP. HLA-DR-restricted T-cell responses to factor VIII epitopes in a mild haemophilia A family with missense substitution A2201P. Haemophilia. May 2010. PMID: 20536985
- Ge X, Tan V, Bollyky PL, Standifer NE, James EA, Kwok WW. Assessment of seasonal influenza A virus-specific CD4 T-cell responses to 2009 pandemic H1N1 swine-origin influenza A virus. J Virol. Apr 2010. PMID: 20071564
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- Long SA, Walker MR, Rieck M, James EA, Kwok WW, Sanda S, Pihoker C, Nepom GT, Buckner JH. Functional islet-specific Treg can be generated from CD4+CD25- T cells of healthy and type 1 diabetic subjects. Eur J Immunol. Feb 2009. PMID: 19180473
- Yang J, James EA, Roti M, Huston L, Gebe JA, Kwok WW. Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals. Int Immunol. Jan 2009. PMID: 19050106
- Kwok WW, Yang J, James EA, Bui J, Huston L, Wiesen AR, Roti M. The anthrax vaccine adsorbed vaccine generates protective antigen (PA)-Specific CD4+ T cells with a phenotype distinct from that of naive PA T cells. Infect Immun. Oct 2008. PMID: 18678674
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- James EA, Kwok WW. Low-affinity major histocompatibility complex-binding peptides in type 1 diabetes. Diabetes. Jul 2008. PMID: 18586912
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- Roti M, Yang J, Berger D, Huston L, James EA, Kwok WW. Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus. J Immunol. Feb 2008. PMID: 18209073
- James EA, Kwok WW, Ettinger RA, Thompson AR, Pratt KP. T-cell responses over time in a mild hemophilia A inhibitor subject: epitope identification and transient immunogenicity of the corresponding self-peptide. J Thromb Haemost. Dec 2007. PMID: 18034765
- James EA, Bui J, Berger D, Huston L, Roti M, Kwok WW. Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition. Int Immunol. Nov 2007. PMID: 17906339
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- James EA, Kwok WW. CD8+ suppressor-mediated regulation of human CD4+ T cell responses to glutamic acid decarboxylase 65. Eur J Immunol. Jan 2007. PMID: 17163450
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- Yang J, Danke NA, Berger D, Reichstetter S, Reijonen H, Greenbaum CJ, Pihoker C, James EA, Kwok WW. Islet-specific glucose-6-phosphatase catalytic subunit-related protein-reactive CD4+ T cells in human subjects. J Immunol. Mar 2006. PMID: 16493034