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Eddie James

Eddie James, PhD profile picture
Director of the Tetramer Lab Core at BRI
Email: 
ejames@benaroyaresearch.org
Phone: 
206-287-5603

Background Information

Dr. James received his bachelor’s degree in Chemical Engineering from the University of Colorado. He subsequently attended Washington State University, receiving his PhD in Biochemical Engineering in 2001. After completing an internship at Zymogenetics, he joined the Benaroya Research Institute as manager of the tetramer core and is currently a BRI assistant member.

Area of Research

Dr. James leads several independent research projects related to the role of T cells in autoimmune disease. These projects include a JDRF funded study to investigate the role of protein modifications in the development of destructive immune responses in type 1 diabetes and a cooperative JDRF project aimed at understanding immunologic reasons why some patients with type 1 diabetes rapidly lose all ability to produce insulin while others retain some residual insulin secretion. He also collaborates with Dr. John Piganelli and Dr. Meghan Marre of the University of Pittsburgh and Dr. Chantal Mathieu and Lut Overbergh to investigate how beta cell stress contributes to the breakdown of tolerance in autoimmune diabetes and with Dr. Bill Kwok to study activated T cells in subjects at risk of developing type 1 diabetes. Dr. James is currently co-chair of the JDRF biomarker working group and co-leads the Immunology of Diabetes Society T Cell Workshop. Under the auspices of these groups, he leads biomarker development and validation projects aimed at improved disease staging of subjects who are diagnosed with type 1 diabetes.

Dr. James participates in collaborative projects with Dr. Jane Buckner and colleagues at the University of Colorado aimed at characterizing T cell responses in patients with rheumatoid arthritis and investigating mechanisms that promote the breakdown of tolerance in at risk subjects. He is also involved in various  other collaborative research projects, applying tetramer technology to identify the chemical patterns recognized by T cells in desirable protective immune responses directed against viruses (such as influenza, tetanus, and chicken pox) and in undesirable destructive immune responses directed against therapeutic proteins (including insulin and Factor VIII) and self-proteins in diseases such as IBD and pemphigus vulgaris. The unifying theme of this work is that understanding the basics of immune recognition and the specifics of both immune protection and immune destruction will lead to better diagnostic tools and better therapies.

Featured Publications

  1. Guyer P, Seminova K, Lugar M, Manganaro A, Velarde de la Cruz EE, Hartley R, Smithmyer ME, Speake C, Bonifacio E, Kent SC, James EA. T cell and autoantibody recognition of nucleus-associated islet autoantigens in individuals with type 1 diabetes. Diabetologia. Jul 2025. PMID: 40490584
  2. Savvides CS, James EA, Buckner JH. Rheumatoid arthritis - the role of T cells in this complex systemic autoimmune disease. Curr Opin Immunol. Apr 2025. PMID: 40209552
  3. Hohenstein AC, Gallegos JB, Dang M, Groegler J, Broncucia H, Tensun FA, Waugh K, Dong F, James EA, Speake C, Steck AK, Rewers MJ, Gottlieb PA, Haskins K, Delong T, Baker RL. Novel T-Cell Reactivities to Hybrid Insulin Peptides in Islet Autoantibody-Positive At-Risk Individuals. Diabetes. Mar 2025. PMID: 39820647
  4. Rims C, Uchtenhagen H, Brooks K, Ng B, Posso SE, Carlin J, Kwok WW, Buckner JH, James EA. Antigen-specific T-cell frequency and phenotype mirrors disease activity in DRB1*04:04+ rheumatoid arthritis patients. Clin Exp Immunol. Nov 2024. PMID: 39492692
  5. Tewari R, Yang SJ, McClain ED, Hu A, Mortensen E, DeSchmidt A, Chen J, Kancharla A, Singh AK, James EA, Burman BE, Siddique A, Rawlings DJ, Patel C, Cerosaletti K, Buckner JH. Identification of a novel PDC-E2 epitope in primary biliary cholangitis: Application for engineered Treg therapy. J Autoimmun. Oct 2024. PMID: 39476446
  6. Holers VM, Demoruelle KM, Buckner JH, James EA, Firestein GS, Robinson WH, Steere AC, Zhang F, Norris JM, Kuhn KA, Deane KD. Distinct mucosal endotypes as initiators and drivers of rheumatoid arthritis. Nat Rev Rheumatol. Sep 2024. PMID: 39251771
  7. Groegler J, Callebaut A, James EA, Delong T. The insulin secretory granule is a hotspot for autoantigen formation in type 1 diabetes. Diabetologia. May 2024. PMID: 38811417
  8. Karataş L, Tatar Z, James EA, Colakogullari M. Investigating Associations between HLA-DR Genotype, H. pylori Infection, and Anti-CagA IgA Seropositivity in a Turkish Gastritis Cohort. Genes (Basel). Mar 2024. PMID: 38540398
  9. Callebaut A, Guyer P, Derua R, Buitinga M, Manganaro A, Yi X, Sodré FMC, Vig S, Suleiman M, Marchetti P, Eizirik DL, Kent SC, Mathieu C, James EA, Overbergh L. CD4+ T cells from individuals with type 1 diabetes respond to a novel class of deamidated peptides formed in pancreatic islets. Diabetes. Feb 2024. PMID: 38387030
  10. Callebaut A, Guyer P, Baker RL, Gallegos JB, Hohenstein AC, Gottlieb PA, Mathieu C, Overbergh L, Haskins K, James EA. An Insulin-Chromogranin A Hybrid Peptide activates DR11 restricted T cells in human type 1 diabetes. Diabetes. Jan 2024. PMID: 38295386
  11. James EA, Holers VM, Iyer R, Prideaux EB, Rao NL, Rims C, Muir VS, Posso SE, Bloom MS, Zia A, Elliott SE, Adamska JZ, Ai R, Brewer RC, Seifert JA, Moss L, Barzideh S, Demoruelle MK, Striebich CC, Okamoto Y, Sainbayar E, Crook AA, Peterson RA, Vanderlinden LA, Wang W, Boyle DL, Robinson WH, Buckner JH, Firestein GS, Deane KD. Multifaceted immune dysregulation characterizes individuals at-risk for rheumatoid arthritis. Nat Commun. Nov 2023. PMID: 37993439
  12. James EA, Joglekar AV, Linnemann AK, Russ HA, Kent SC. The Beta Cell-Immune Cell Interface in Type 1 Diabetes (T1D). Mol Metab. Sep 2023. PMID: 37734713
  13. James EA. Editorial: Processing, presentation, and recognition of T cell determinants: From molecular insights to clinical applications. Cell Immunol. Aug 2023. PMID: 37603955
  14. Ettinger RA, Buitinga M, Vandamme C, Afonso G, Gomez R, Arribas-Layton D, Bissenova S, Speake C, Reijonen H, Kinnunen T, Overbergh L, Mallone R, Kwok WW, James EA. Technical Validation and Utility of an HLA Class II Tetramer Assay for Type 1 Diabetes: A Multicenter Study. J Clin Endocrinol Metab. Jul 2023. PMID: 37474341
  15. Moustakas AK, Nguyen H, James EA, Papadopoulos GK. Autoimmune susceptible HLA class II motifs facilitate the presentation of modified neoepitopes to potentially autoreactive T cells. Cell Immunol. May 2023. PMID: 37301094
  16. Moon JS, Younis S, Ramadoss NS, Iyer R, Sheth K, Sharpe O, Rao NL, Becart S, Carman JA, James EA, Buckner JH, Deane KD, Holers VM, Goodman SM, Donlin LT, Davis MM, Robinson WH. Cytotoxic CD8(+) T cells target citrullinated antigens in rheumatoid arthritis. Nat Commun. Jan 2023. PMID: 36658110
  17. Nguyen H, Arribas-Layton D, Chow IT, Speake C, Kwok WW, Hessner MJ, Greenbaum CJ, James EA. Characterizing T cell responses to enzymatically modified beta cell neo-epitopes. Front Immunol. Jan 2023. PMID: 36703975
  18. Chriswell ME, Lefferts AR, Clay MR, Hsu AR, Seifert J, Feser ML, Rims C, Bloom MS, Bemis EA, Liu S, Maerz MD, Frank DN, Demoruelle MK, Deane KD, James EA, Buckner JH, Robinson WH, Holers VM, Kuhn KA. Clonal IgA and IgG autoantibodies from individuals at risk for rheumatoid arthritis identify an arthritogenic strain of Subdoligranulum. Sci Transl Med. Oct 2022. PMID: 36288282
  19. Strollo R, Vinci C, Man YKS, Bruzzaniti S, Piemonte E, Alhamar G, Briganti SI, Malandrucco I, Tramontana F, Fanali C, Garnett J, Buccafusca R, Guyer P, Mamula M, James EA, Pozzilli P, Ludvigsson J, Winyard PG, Galgani M, Nissim A. Autoantibody and T cell responses to oxidative post-translationally modified insulin neoantigenic peptides in type 1 diabetes. Diabetologia. Oct 2022. PMID: 36207582
  20. Guyer P, Arribas-Layton D, Manganaro A, Speake C, Lord S, Eizirik DL, Kent SC, Mallone R, James EA. Recognition of mRNA Splice Variant and Secretory Granule Epitopes by CD4+ T Cells in Type 1 Diabetes. Diabetes. Oct 2022. PMID: 36201618
  21. Alvarez I, Antón LC, James EA. Editorial: alternative antigen processing and presentation in immune disorders. Front Immunol. Sep 2022. PMID: 36159790
  22. Holers VM, Kuhn KA, Demoruelle MK, Norris JM, Firestein GS, James EA, Robinson WH, Buckner JH, Deane KD. Mechanism-driven strategies for prevention of rheumatoid arthritis. Rheumatol Autoimmun. Sep 2022. PMID: 36312783
  23. Yang ML, Connolly SE, Gee RJ, Lam TT, Kanyo J, Peng J, Guyer P, Syed F, Tse HM, Clarke SG, Clarke CF, James EA, Speake C, Evans-Molina C, Arvan P, Herold KC, Wen L, Mamula MJ. Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. Diabetes. Sep 2022. PMID: 35730902
  24. Chen D, McInnis E, Wu E, Stember K, Hogan S, Hu Y, Henderson C, Blazek L, Mallal S, Karosiene E, Peters B, Sidney J, James EA, Kwok WW, Jennette JC, Ciavatta DJ, Falk RJ, Free ME. Immunological Interaction of HLA-DPB1 and Proteinase 3 in ANCA Vasculitis is Associated with Clinical Disease Activity. J Am Soc Nephrol. Jun 2022. PMID: 35672132
  25. Maggi J, Carrascal M, Soto L, Neira O, Cuéllar MC, Aravena O, James EA, Abian J, Jaraquemada D, Catalan D, Aguillón JC. Isolation of HLA-DR-naturally presented peptides identifies T-cell epitopes for rheumatoid arthritis. Ann Rheum Dis. Apr 2022. PMID: 35459695
  26. Yang ML, Horstman S, Gee R, Guyer P, Lam TT, Kanyo J, Perdigoto AL, Speake C, Greenbaum CJ, Callebaut A, Overbergh L, Kibbey RG, Herold KC, James EA, Mamula MJ. Citrullination of glucokinase is linked to autoimmune diabetes. Nat Commun. Apr 2022. PMID: 35388005
  27. Ishigaki K, Lagattuta KA, Luo Y, James EA, Buckner JH, Raychaudhuri S. HLA autoimmune risk alleles restrict the hypervariable region of T cell receptors. Nat Genet. Mar 2022. PMID: 35332318
  28. Jing Y, Kong Y, McGinty J, Blahnik-Fagan G, Lee T, Orozco-Figueroa S, Bettini ML, James EA, Bettini M. TCR/HLA Humanized Mice Reveal Reduced Tolerance and Increased Immunogenicity of Post-translationally Modified GAD65 Epitope. Diabetes. Feb 2022. PMID: 35179565
  29. Yang JHM, Ward-Hartstonge KA, Perry DJ, Blanchfield JL, Posgai AL, Wiedeman AE, Diggins K, Rahman A, Tree TIM, Brusko TM, Levings MK, James EA, Kent SC, Speake C, Homann D, Long SA, Immunology of Diabetes Society T-Cell Cytometry Group.. Guidelines for standardizing T-cell cytometry assays to link biomarkers, mechanisms, and disease outcomes in type 1 diabetes. Eur J Immunol. Jan 2022. PMID: 35025103
  30. Hill DL, Whyte CE, Innocentin S, Lee JL, Dooley J, Wang J, James EA, Lee JC, Kwok WW, Zand MS, Liston A, Carr EJ, Linterman MA. Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans. Elife. Nov 2021. PMID: 34726156
  31. Rodriguez-Calvo T, Christoffersson G, Bender C, von Herrath MG, Mallone R, Kent SC, James EA. Means, Motive, and Opportunity: Do Non-Islet-Reactive Infiltrating T Cells Contribute to Autoimmunity in Type 1 Diabetes? Front Immunol. Jun 2021. PMID: 34220832)
  32. Wiles TA, Hohenstein A, Landry LG, Dang M, Powell R, Guyer P, James EA, Nakayama M, Haskins K, Delong T, Baker RL. Characterization of Human CD4 T Cells Specific for a C-Peptide/C-Peptide Hybrid Insulin Peptide. Front Immunol. May 2021. PMID: 34113344
  33. Song J, Schwenzer A, Wong A, Turcinov S, Rims C, Martinez LR, Arribas-Layton D, Gerstner C, Muir VS, Midwood KS, Malmström V, James EA, Buckner JH. Shared recognition of citrullinated tenascin-C peptides by T and B cells in rheumatoid arthritis. JCI Insight. Mar 2021. PMID: 33507879
  34. Piganelli JD, Mamula MJ, James EA. The Role of β Cell Stress and Neo-Epitopes in the Immunopathology of Type 1 Diabetes. Front Endocrinol (Lausanne). Feb 2021. PMID: 33679609
  35. Nguyen H, Guyer P, Ettinger RA, James EA. Non-Genetically Encoded Epitopes Are Relevant Targets in Autoimmune Diabetes. Biomedicines. Feb 2021. PMID: 33671312
  36. DeGottardi Q, Gates TJ, Yang J, James EA, Malhotra U, Chow IT, Simoni Y, Fehlings M, Newell EW, DeBerg HA, Kwok WW. Ontogeny of different subsets of yellow fever virus-specific circulatory CXCR5+ CD4+ T cells after yellow fever vaccination. Sci Rep. Sep 2020. PMID: 32973217
  37. Carmona-Rivera C, Carlucci PM, Goel RR, James EA, Brooks SR, Rims C, Hoffmann V, Fox DA, Buckner JH, Kaplan MJ. Neutrophil extracellular traps mediate articular cartilage damage and enhance cartilage component immunogenicity in rheumatoid arthritis. JCI Insight. Jul 2020. PMID: 32484790
  38. James EA, Mallone R, Kent SC, DiLorenzo TP. T-Cell Epitopes and Neo-epitopes in Type 1 Diabetes: A Comprehensive Update and Reappraisal. Diabetes. Jul 2020. PMID: 32561620
  39. Arribas-Layton D, Guyer P, Delong T, Dang M, Chow IT, Speake C, Greenbaum CJ, Kwok WW, Baker RL, Haskins K, James EA. Hybrid Insulin Peptides Are Recognized by Human T Cells in the Context of DRB1*04:01. Diabetes. Jul 2020. PMID: 32291282
  40. Larson D, Patel P, Salapatek AM, Couroux P, Whitehouse D, Pina A, Johnson JL, Sever ML, Sanda S, Poyser J, Allio T, Scadding GW, Qin T, Shamji MH, Kwok WW, James EA, French D, Lelic A, Larché M, Altman MC, Togias A, Durham SR. Nasal allergen challenge and environmental exposure chamber challenge: A randomized trial comparing clinical and biological responses to cat allergen. J Allergy Clin Immunol. Jun 2020. PMID: 32169380
  41. Abdelsamed HA, Zebley CC, Nguyen H, Rutishauser RL, Fan Y, Ghoneim HE, Crawford JC, Alfei F, Alli S, Ribeiro SP, Castellaw AH, McGargill MA, Jin H, Boi SK, Speake C, Serti E, Turka LA, Busch ME, Stone M, Deeks SG, Sekaly RP, Zehn D, James EA, Nepom GT, Youngblood B. Beta cell-specific CD8+ T cells maintain stem cell memory-associated epigenetic programs during type 1 diabetes. Nat Immunol. May 2020. PMID: 32231298
  42. Lande R, Palazzo R, Gestermann N, Jandus C, Falchi M, Spadaro F, Riccieri V, James EA, Butera A, Boirivant M, Feldmeyer L, Surbeck I, Di Lucca J, Stuber F, Spinelli FR, Botti E, Marinari B, Bianchi L, Pica R, Cerbelli B, Giannakakis K, Auteri SE, Daniels I, Durrant LG, Horstman S, Costanzo A, Romero P, Alessandri C, Conti F, Valesini G, Gilliet M, Chizzolini C, Frasca L. Native/citrullinated LL37-specific T-cells help autoantibody production in Systemic Lupus Erythematosus. Sci Rep. Apr 2020. PMID: 32245990
  43. Wen X, Yang J, James EA, Chow IT , Reijonen H , Kwok WW. Increased islet antigen-specific regulatory and effector CD4+ T cells in healthy individuals with the type 1 diabetes-protective haplotype. Sci Immunol. Feb 2020. PMID: 32060144
  44. Wiedeman AE, Muir VS, Rosasco MG, DeBerg HA, Presnell S, Haas B, Dufort MJ, Speake C, Greenbaum CJ, Serti E, Nepom GT, Blahnik G, Kus AM, James EA, Linsley PS, Long SA. Autoreactive CD8+ T cell exhaustion distinguishes subjects with slow type 1 diabetes progression. J Clin Invest. Jan 2020. PMID: 31815738
  45. Free ME, Stember KG, Hess JJ, McInnis EA, Lardinois O, Hogan SL, Hu Y, Mendoza C, Le AK, Guseman AJ, Pilkinton MA, Bortone DS, Cowens K, Sidney J, Karosiene E, Peters B, James EA, Kwok WW, Vincent BG, Mallal SA, Jennette JC, Ciavatta DJ, Falk RJ. Restricted myeloperoxidase epitopes drive the adaptive immune response in MPO-ANCA vasculitis. J Autoimmun. Jan 2020. PMID: 31383567
  46. Uchida AM, Boden EK, James EA, Shows DM, Konecny AJ, Lord JD. Escherichiacoli-Specific CD4+ T Cells Have Public T-Cell Receptors and Low Interleukin 10 Production in Crohn's Disease. Cell Mol Gastroenterol Hepatol. Jan 2020. PMID: 32361018
  47. Galindo-Feria AS, Albrecht I, Fernandes-Cerqueira C, Notarnicola A, James EA, Herrath J, Dastmalchi M, Sandalova T, Rönnblom L, Jakobsson PJ, Fathi M, Achour A, Grunewald J, Malmström V, Lundberg IE. Proinflammatory Histidyl-Transfer RNA Synthetase-Specific CD4+ T Cells in the Blood and Lungs of Patients With Idiopathic Inflammatory Myopathies. Arthritis Rheumatol. Jan 2020. PMID: 31403245
  48. Speake C, Skinner SO, Berel D, Whalen E, Dufort MJ, Young WC, Odegard JM, Pesenacker AM, Gorus FK, James EA, Levings MK, Linsley PS, Akirav EM, Pugliese A, Hessner MJ, Nepom GT, Gottardo R, Long SA. A composite immune signature parallels disease progression across T1D subjects. JCI Insight. Dec 2019. PMID: 31671072
  49. Rudulier CD, Tonti E, James EA, Kwok WW, Larché M. Modulation of CRTh2 expression on allergen-specific T cells following peptide immunotherapy. Allergy. Nov 2019. PMID: 31077596
  50. Longino NV, Yang J, Iyer JG, Ibrani D, Chow IT, Laing KJ, Campbell VL, Paulson KG, Kulikauskas RM, Church CD, James EA, Nghiem P, Kwok WW, Koelle DM. Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain. Cancer Immunol Res. Oct 2019. PMID: 31405946
  51. Meckiff BJ, Ladell K, McLaren JE, Ryan GB, Leese AM, James EA, Price DA, Long HM. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. Sep 2019. PMID: 31308093
  52. Chow IT, Gates TJ, Papadopoulos GK, Moustakas AK, Kolawole EM, Notturno RJ, McGinty JW, Torres-Chinn N, James EA, Greenbaum CJ, Nepom GT, Kwok WW. Discriminative T cell recognition of cross-reactive islet-antigens is associated with HLA-DQ8 transdimer-mediated autoimmune diabetes. Sci Adv. Aug 2019. PMID: 31457096
  53. Ahmed S, Cerosaletti K, James EA, Long SA, Mannering SI, Speake C, Nakayama M, Tree T, Roep BO, Herold KC, Brusko TM. Standardizing T-Cell Biomarkers in Type 1 Diabetes: Challenges and Recent Advances. Diabetes. Jul 2019. PMID: 31221801
  54. Rims C, Uchtenhagen H, Kaplan MJ, Carmona-Rivera C, Carlucci P, Mikecz K, Markovics A, Carlin J, Buckner JH, James EA. Citrullinated Aggrecan Epitopes as Targets of Autoreactive CD4+ T Cells in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. Apr 2019. PMID: 30390384
  55. Blahnik G, Uchtenhagen H, Chow IT, Speake C, Greenbaum CJ, Kwok WW, James EA. Analysis of pancreatic beta cell specific CD4+ T cells reveals a predominance of proinsulin specific cells. Cell Immunol. Jan 2019. PMID: 30428974
  56. James EA, Gillette L, Durinovic-Belló I, Speake C, Bondinas GP, Moustakas AK, Greenbaum CJ, Papadopoulos GK, Kwok WW. DRB4*01:01 Has a Distinct Motif and Presents a Proinsulin Epitope That Is Recognized in Subjects with Type 1 Diabetes. J Immunol. Dec 2018. PMID: 30455401
  57. Yang J, Jing L, James EA, Gebe JA, Koelle DM, Kwok WW. A Novel Approach of Identifying Immunodominant Self and Viral Antigen Cross-Reactive T Cells and Defining the Epitopes They Recognize. Front Immunol. Dec 2018. PMID: 30619245
  58. Buitinga M, Callebaut A, Marques Câmara Sodré F, Crèvecoeur I, Blahnik-Fagan G, Yang ML, Bugliani M, Arribas-Layton D, Marré M, Cook DP, Waelkens E, Mallone R, Piganelli JD, Marchetti P, Mamula MJ, Derua R, James EA, Mathieu C, Overbergh L. Inflammation-Induced Citrullinated Glucose-Regulated Protein 78 Elicits Immune Responses in Human Type 1 Diabetes. Diabetes. Nov 2018. PMID: 30348823
  59. Birrueta G, Tripple V, Pham J, Manohar M, James EA, Kwok WW, Nadeau KC, Sette A, Peters B, Schulten V. Peanut-specific T cell responses in patients with different clinical reactivity. PLoS One. Oct 2018. PMID: 30304054
  60. Kimura T, Kobiyama K, Winkels H, Tse K, Miller J, Vassallo M, Wolf D, Ryden C, Orecchioni M, Dileepan T, Jenkins MK, James EA, Kwok WW, Hanna DB, Kaplan RC, Strickler HD, Durkin HG, Kassaye SG, Karim R, Tien PC, Landay AL, Gange SJ, Sidney J, Sette A, Ley K. Regulatory CD4+ T Cells Recognize Major Histocompatibility Complex Class II Molecule-Restricted Peptide Epitopes of Apolipoprotein B. Circulation. Sep 2018. PMID: 29588316
  61. Marré ML, Piganelli JD, James EA. Protecting functional β cells with a therapeutic peptide. Ann Transl Med. Sep 2018. PMID: 30370299
  62. Pieper J, Dubnovitsky A, Gerstner C, James EA, Rieck M, Kozhukh G, Tandre K, Pellegrino S, Gebe JA, Rönnblom L, Sandalova T, Kwok WW, Klareskog L, Buckner JH, Achour A, Malmström V. Memory T cells specific to citrullinated α-enolase are enriched in the rheumatic joint. J Autoimmun. Aug 2018. PMID: 29853344
  63. Marré ML, McGinty JW, Chow IT, DeNicola ME, Beck NW, Kent SC, Powers AC, Bottino R, Harlan DM, Greenbaum CJ, Kwok WW, Piganelli JD, James EA. Modifying Enzymes Are Elicited by ER Stress, Generating Epitopes That Are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes. Diabetes. Jul 2018. PMID: 29654212
  64. James EA, Pietropaolo M, Mamula MJ. Immune Recognition of β-Cells: Neoepitopes as Key Players in the Loss of Tolerance. Diabetes. Jun 2018. PMID: 29784651
  65. James EA, Abreu JRF, McGinty JW, Odegard JM, Fillié YE, Hocter CN, Culina S, Ladell K, Price DA, Alkanani A, Rihanek M, Fitzgerald-Miller L, Skowera A, Speake C, Gottlieb P, Davidson HW, Wong FS, Roep B, Mallone R, Immunology of Diabetes Society T Cell Workshop Committee.. Combinatorial detection of autoreactive CD8+ T cells with HLA-A2 multimers: a multi-centre study by the Immunology of Diabetes Society T Cell Workshop. Diabetologia. Mar 2018. PMID: 29196783
  66. Ettinger RA, Liberman JA, Gunasekera D, Puranik K, James EA, Thompson AR, Pratt KP. FVIII proteins with a modified immunodominant T-cell epitope exhibit reduced immunogenicity and normal FVIII activity. Blood Adv. Feb 2018. PMID: 29444872
  67. Reynolds CJ, Suleyman OM, Ortega-Prieto AM, Skelton JK, Bonnesoeur P, Blohm A, Carregaro V, Silva JS, James EA, Maillère B, Dorner M, Boyton RJ, Altmann DM. T cell immunity to Zika virus targets immunodominant epitopes that show cross-reactivity with other Flaviviruses. Sci Rep. Jan 2018. PMID: 29330423
  68. James EA. Direct Analysis of Insulin-Specific T Cells Provides New Insights. Diabetes. Dec 2017. PMID: 29162735
  69. Sprouse ML, Blahnik G, Lee T, Tully N, Benarjee P, James EA, Redondo MJ, Bettini ML, Bettini M. Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs. J Vis Exp. Sep 2017. PMID: 28930975
  70. Scholz EM, Marcilla M, Daura X, Arribas-Layton D, James EA, Alvarez I. Human Leukocyte Antigen (HLA)-DRB1*15:01 and HLA-DRB5*01:01 Present Complementary Peptide Repertoires. Front Immunol. Aug 2017. PMID: 28871256
  71. Acevedo-Calado M, James EA, Morran MP, Pietropaolo SL, Ouyang Q, Arribas-Layton D, Songini M, Liguori M, Casu A, Auchus RJ, Huang S, Yu L, Michels A, Gianani R, Pietropaolo M. Identification of Unique Antigenic Determinants in the Amino Terminus of IA-2 (ICA512) in Childhood and Adult Autoimmune Diabetes: New Biomarker Development. Diabetes Care. Apr 2017. PMID: 28174261
  72. Carmona-Rivera C, Carlucci PM, Moore E, Lingampalli N, Uchtenhagen H, James EA, Liu Y, Bicker KL, Wahamaa H, Hoffmann V, Catrina AI, Thompson PR, Buckner JH, Robinson WH, Fox DA, Kaplan MJ. Synovial fibroblast-neutrophil interactions promote pathogenic adaptive immunity in rheumatoid arthritis. Sci Immunol. Apr 2017. PMID: 28649674
  73. Sprouse ML, Blahnik G, Lee T, Tully N, Banerjee P, James EA, Redondo MJ, Bettini ML, Bettini M. Rapid identification and expression of human TCRs in retrogenic mice. J Immunol Methods. Dec 2016. PMID: 27589924
  74. Babon JA, DeNicola ME, Blodgett DM, Crèvecoeur I, Buttrick TS, Maehr R, Bottino R, Naji A, Kaddis J, Elyaman W, James EA, Haliyur R, Brissova M, Overbergh L, Mathieu C, Delong T, Haskins K, Pugliese A, Campbell-Thompson M, Mathews C, Atkinson MA, Powers AC, Harlan DM, Kent SC. Analysis of self-antigen specificity of islet-infiltrating T cells from human donors with type 1 diabetes. Nat Med. Dec 2016. PMID: 27798614
  75. Gerstner C, Dubnovitsky A, Sandin C, Kozhukh G, Uchtenhagen H, James EA, Rönnelid J, Ytterberg AJ, Pieper J, Reed E, Tandre K, Rieck M, Zubarev RA, Rönnblom L, Sandalova T, Buckner JH, Achour A, Malmström V. Functional and Structural Characterization of a Novel HLA-DRB1*04:01-Restricted α-Enolase T Cell Epitope in Rheumatoid Arthritis. Front Immunol. Nov 2016. PMID: 27895642
  76. Ettinger RA, Paz P, James EA, Gunasekera D, Aswad F, Thompson AR, Matthews DC, Pratt KP. T cells from hemophilia A subjects recognize the same HLA-restricted FVIII epitope with a narrow TCR repertoire. Blood. Oct 2016. PMID: 27471234
  77. Scholz E, Mestre-Ferrer A, Daura X, García-Medel N, Carrascal M, James EA, Kwok WW, Canals F, Alvarez I. A Comparative Analysis of the Peptide Repertoires of HLA-DR Molecules Differentially Associated With Rheumatoid Arthritis. Arthritis Rheumatol. Oct 2016. PMID: 27158783
  78. Nguyen H, James EA. Immune recognition of citrullinated epitopes. Immunology. Oct 2016. PMID: 27531825
  79. Uchtenhagen H, Rims C, Blahnik G, Chow IT, Kwok WW, Buckner JH, James EA. Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining. Nat Commun. Aug 2016. PMID: 27571776
  80. Hinz D, Seumois G, Gholami AM, Greenbaum JA, Lane J, White B, Broide DH, Schulten V, Sidney J, Bakhru P, Oseroff C, Wambre E, James EA, Kwok WW, Peters B, Vijayanand P, Sette A. Lack of allergy to timothy grass pollen is not a passive phenomenon but associated with the allergen-specific modulation of immune reactivity. Clin Exp Allergy. May 2016. PMID: 26662458
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